| 1. | The recornbinant vector pcambia 1305 . 1 containing s gene was confirmed by double digestion of restriction endonuclease
经双酶切鉴定证实s基因克隆到载体pcambia1305 . 1上。 |
| 2. | The positive clone was screened and identified by pcr and analysis of restriction endonuclease digestion
转化感受态大肠杆菌dh5 ，经pcr及限制性内切酶分析得到阳性克隆。 |
| 3. | 3 . pbv220 - endostatin was transformed into e . coli dh5a . the positive colony was screened and identified by pcr and restriction endonuclease digestion
3 .将重组质粒转化到克隆菌dh5a中，经pcr筛选和限制性内切酶鉴定，得到阳性克隆菌株。 |
| 4. | The genome could be digested only by restriction endonucleases hind , accl and xbal , and was resistant to cleavage by a wide range of other restriction endonucleases
病毒的基因组可以被hind 、 acc和xba酶切消化，但不能被其他所使用的限制性内切酶消化。 |
| 5. | The genomic dna of ginkgo extracted by ctab method is light brown in color . it is neither digested with restriction endonucleases nor acted as template for polymerase chain reaction ( pcr )
常规的ctab法提取的银杏基因组dna呈黄色，不易被内切酶所消化，也不能作为pcr模板。 |
| 6. | E2 gene was cut with restriction endonuclease from the recombination plasmid t - e2 , and subcloned into the expression vector ppic9 ' s mcs , which was identified by enzyme digestion and pcr amplification
将重组质粒t - e2的e2基因酶切后，亚克隆到表达载体ppic9上，经酶切和pcr鉴定，命名为ppic9 - e2 。 |
| 7. | The recombinant vector pbi121 containing si gene of ibv was confirmed by pcr and double digestion of restriction endonuclease . agrobacterium fumefaciens eha105 with the recombinant vector pbi121 was obtained by tri - parental mating method
用三亲交配法将重组克隆导入根癌农杆菌，获得了含pbi121重组质粒的eha105农杆菌菌株。 |
| 8. | The fragments digested with restriction endonucleases ecor i and hind iii were subsequently cloned into the vector pkk223 . 3 . e . coli jm 109 transformed with the expression plasmid was grown in m9 media to an optical density of 0 . 7
把重组质粒转化入大肠杆菌jm109中，挑取转化成功的单克隆菌，接种到含amp的lb培养基中，于37振摇过夜。 |
| 9. | 713bp and 700bp specific fragments were amplified by pcr and ligated into pgem - t easy vector . it was identified by restriction endonuclease digest analysis , pcr and sequencing that this fragment contained the complete open reading frame ( orf ) of the hc and ha gene
扩增产物连接到pgem - teasy载体上，转化入大肠杆菌jm109中进行蓝白斑筛选后，用酶切、 pcr鉴定和测序的方法鉴定出重组阳性质粒( pgem - hc和pgem - ha ) 。 |
| 10. | Based on the restriction endonuclease sites of the reference sequences of a , b , e subgroups published in ncbi , the dna was digested by restriction endonucleases bgiii , bamhi , sspi . the expected results were obtained when digested by bgiii specific to a subgroup or sspi specific to a and e subgroups
结果发现，扩增到的目的带只能用对a亚群特异的bgl限制酶或对a 、 e亚群特异的ssp限制酶切开，而不能用对b亚群特异的bamh限制酶切开。 |
